Báo cáo Development of an Improved Capability in support of National Bio-Security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs

Ministry of Agriculture & Rural Development Progress Report Development of an Improved Capability in support of National Bio-security for the Surveillance and Control of Foot & Mouth Disease in Cattle and Pigs. Date 30/06/2006 Table of Contents 1. Institute Information_____________________________________________ 3 2. Project Abstract ________________________________________________ 4 3. Executive Summary _____________________________________________ 4 4. Introduction & Background _______________________________________ 5 5. Progress to Date________________________________________________ 6 5.1 Implementation Highlights ____________________________________ 6 5.2 Smallholder Benefits_________________________________________ 7 5.3 Capacity Building ___________________________________________ 8 5.4 Publicity ___________________________________________________ 8 5.5 Project Management _________________________________________ 8 6. Report on Cross-Cutting Issues ___________________________________ 8 6.1 Environment _______________________________________________ 8 6.2 Gender and Social Issues_____________________________________ 8 7. Implementation & Sustainability Issues_____________________________ 9 7.1 Issues and Constraints_______________________________________ 9 7.2 Options____________________________________________________ 9 7.3 Sustainability _______________________________________________ 9 8. Next Critical Steps _____________________________________________ 10 9. Conclusion ___________________________________________________ 10 10. Statuatory Declaration ________________________________________ 13 1. Institute Information Project Name Vietnamese Institution Regional Animal Health Centre, Ho Chi Minh City (RAHC-HCMC), South Vietnam. Vietnamese Project Team Leader Dr. Dong Manh Hoa Australian Organisation Australian Animal Health Laboratory (AAHL), PMB 24, Geelong, 3213, Australia Australian Personnel Mr Chris Morrissy Date commenced 01/06/2005 Completion date (original) 01/06/2008 Completion date (revised) Reporting period 31/12/2005 – 30/06/2006 Contact Officer(s) In Australia: Team Leader Name: Mr Chris Morrissy Telephone: +61 3 5227 5000 Position: Diagnostic Virologist Supervisor Mammalian Virology Fax: +61 3 5227 5555 Organisation Australian Animal Health Laboratory (AAHL), PMB 24, Geelong, 3213, Australia Email: chris.morrissy@csiro.au In Australia: Administrative contact Name: Mr Chris Morrissy Telephone: +61 3 5227 5000 Position: Patents Contracts Officer Fax: +61 3 5227 5555 Organisation Australian Animal Health Laboratory (AAHL), PMB 24, Geelong, 3213, Australia Email: chris.morrissy@csiro.au In Vietnam Name: Dr. Dong Manh Hoa Telephone: + 84 8 8568220 Position: Director Fax: + 84 8 8569050 Organisation Regional Animal Health Centre, Ho Chi Minh City (RAHC-HCMC), South Vietnam. Email: rahchcmc@hcm.vnn.vn 2. Project Abstract The projects purpose is to determine why there are vaccine failures, and to investigate what serotypes of FMDV are circulating in Vietnam so that the correct vaccines can be used for these serotypes. Regional laboratories are setup with the reagents and methods to allow a diagnostic capability for FMDV diagnosis and serology. Control strategies for understanding of FMD epidemiology have been implemented through veterinary and laboratory training workshops and laboratory testing for both virus typing and serosurveillance has begun. The project has highlighted the importance of having a laboratory network to identify what is happening in the field and how to prevent and control disease outbreaks. The pilot zones are established in provinces near the borders of Vietnam to study serotypes circulating in Vietnam and to determine their origin. The quality and collection of samples have increased giving more data on the FMD situation in Vietnam. Molecular epidemiological studies of the FMDV isolates in these provinces will provide insights to the effectiveness of border control and the origin of FMDV circulating in Vietnam each year. A diagnostic capacity for FMD would allow the early detection and identification of disease enabling better control of disease and help reduce loss of livestock and so productivity. 3. Executive Summary The main focus of this reporting period was to commence the project, begin the training of the field veterinarians in the South, Centre and North of Vietnam and also the training of laboratory staff. All laboratory equipment was purchased and supplied to the laboratories in Vietnam. All laboratory consumables and reagents were also supplied to the laboratories in Vietnam for the first 6 months of the project. AAHL staff prepared the information necessary for the training of field veterinarians. This workshop information and the lectures were used to run workshops for the field veterinarians in the South and Centre of Vietnam and this information was then transferred to Dr Hoa and the staff at Ho Chi Minh City Regional Laboratory who gave the third workshop in Hanoi. The field studies were planned and implemented through the veterinary workshops, the field studies began with the first sero- surveillance survey in the South and Centre of Vietnam and the collection of outbreak samples. The Regional Animal Health Centre in Ho Chi Minh City also visited each province to train the field veterinarians on how to collect samples and how to collect information necessary for the history of the animals on the villages in the project. One staff member from the Regional Animal Health laboratory and one staff member from NAVETCO attended AAHL for training in ELISA technology for the diagnosis of FMD and standardisation of the reagents to be used in the first year of the project. Two AAHL staff consultants carried out workshops for the field veterinarians in the South and Centre of Vietnam. The third veterinary workshop in North Vietnam was carried out by DAH staff. Two AAHL consultants also carried out a workshop for 20 participants from each of the laboratories in the project and the regional laboratories in South Vietnam, in ELISA technologies for diagnosis of FMD at the Regional Animal Health Centre in Ho Chi Minh City. An important part of the laboratory training was Quality Assurance in the laboratory to ensure tests will be run according to a standard protocol and to allow AAHL to audit the results from each laboratory and give the appropriate data to allow trouble shooting. Avian Influenza (AI) has extended the sero-surveillance in the first six months. 4. Introduction & Background Objectives of the project: 1. To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance. 2. To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies. Completing these objectives will improve the diagnostic capability of the Veterinary laboratories in Vietnam and the training of DAH veterinarians in disease investigation and control. This will strengthen the profile of DAH which will play a vital role in making Vietnam more economically competitive. Improved animal health will lead to an increase in rural productivity though increased animal production and indirectly in increased crop production. Healthy animals will enable small farmers to be more competitive in the local market. Control of FMD and animal diseases in general will give poor farmers a more stable income stream and reduce their vulnerability to natural and economic problems. Establishing a diagnostic network which extends from the North to South Vietnam, from the laboratory to the farm level, reinforced by training and education, will give Vietnam a working model on which to base disease control. This will directly increase the competitiveness and productivity of the national agricultural system which includes the major areas of concern including the Mekong Delta and the Central Coast. Implementation Approach and Strategy The project approach is thought to be the most appropriate for developing an understanding of FMD epidemiology in Vietnam. The approach for technology transfer is well established at AAHL and has been successfully applied in previous projects in Vietnam, Thailand and Indonesia. The field studies and epidemiology and serosurveillance approaches have been designed and planned in conjunction with DAH to provide the maximum necessary information to demonstrate the FMD situation in Vietnam and the effectiveness of FMD vaccines. The diagnostic technologies that will be used in this approach are the standard diagnostic tests in use throughout the world to study FMD as directed by OIE. AAHL has a lot of experience with field surveys for prevalence of antibodies, as in the ACIAR projects in Laos and Thailand on FMD. The Philippines is another example where OIE standard diagnostic tests are being used to control and eradicate FMD. 5. Progress to Date 5.1 Implementation Highlights Main achievements for this reporting period were: • Training of laboratory staff at AAHL, one representative from each laboratory were trained in ELISA techniques for the detection of FMD antigen and antibody and the standardisation of reagents. Training was carried out under a quality system emphasizing the importance of Quality Assurance in the laboratory focusing on test maintenance, record keeping and data collection. • A consultant from AAHL completed training of HCMC-RAHC staff in cell culture techniques and established this technology there to be used to grow FMD isolates from the field. The growth of FMD isolates from the field is important to allow further analysis of FMD field isolates by PCR and sequencing, Again Quality Assurance, record keeping and data collection was emphasized. • Further training of field veterinarians and feedback of the results from first round of testing and shared the experience from the first round of sample collection with the field veterinary staff to increase the quality of collected samples. • A consultant from AAHL visited each laboratory to complete the transfer of ELISA technology to each laboratory and to supply the ELISA reagents. Implementation of a Quality Assurance system into the laboratory and the testing of sera in each laboratory was discussed. Training of staf at RAHC- HCMC in the use of molecular techniques for the detection of FMD and the sequencing of FMD isolates. Training of RAHC-HCMC staff in FMD serology using cell culture to carry out the Virus Neutralisation Test (VNT) • Sampling for second phase of sera collection completed for each province for epidemiological studies and serosurveillance. The collection of tissue samples from outbreaks continued to increase giving valuable information on the serotypes circulating in Vietnam • ELISA technology in use at collaborating laboratories in HCMC, Hanoi and Can Tho, for serotyping of FMD tissue samples from the field. Sera from first round of serosurveillance tested at RAHC-HCMC. • Virus isolation in use at RAHC-HCMC. Molecular technology and serology by VNT using cell culture has began to be used. • Supply of consumables and further reagents for testing of samples by FMD ELISA, molecular technology and cell culture. Report from Vietnam: Activities from Jan 1st 2006 to May 30th 2006 1. Training No. Date & place Subject Person in charge Remark 1 02-18 Feb 06 HCM city 1. Tissue culture ( BHK 21, BHK vacc, PK15 , BTY, Pig kidney ,Lamb kidney, Lamb testis ) 2. Using the above cells to isolate FMDV in Vietnam and compare the sensitivity between the cells Ms. Catherine William . Dr. Long Dr.Phong Dr.Vu 4 participants 2 30 March to 15 April 06 AAHL 1. Application of ELISA for FMDV diagnosis (antigen and antibody detection , Ag-ELISA 3ABC-ELISA, LP-ELISA, C- ELISA ) 2. Operation under a Quality Assurance system 3. Reagent standardisation Mr. Chris Morrissy Ms. Linda 5 participants from 4 partner laboratories (NVDC, RAHC- HCMC, CAN THO , DA NANG ) and Sub-DAH- HCMC. 2. Field activities 2.1 Visit 8 provinces in the project (Middle and South Vietnam), March 2006. - Transfer the equipment and documents (form and questionnaire) for sample collection. - Transfer money for sample collection. - Feed back the results from first testing round and share the experience on sample collection with the field veterinary staff to increase the quality of collected samples. - Staff in charge: Dr. Hoa, Dr. Vu , Dr. Ha ( RAHC-HCMC) Dr. Quan, Dr.Quang (RAHC-Da Nang) Dr. Thanh, Dr. Dung (RAHC-Can Tho) 2.1.2 Visit 2 provinces in the project (North Vietnam), March 2006. - Transfer the equipment and documents (form and questionnaire) for sample collection. - Transfer money for sample collection. - Feed back the results from first testing round and share the experience on sample collection with the field veterinary staff to increase the quality of collected samples. - Staff in charge: Dr. Cam, Dr. Tung (NVDC- Hanoi) 2.1.3 Visit 3 laboratories in the Project ( NVDC-Ha Noi , RAHC-Can tho, Da nang) early May, 2006. - Transfer the ELISA reagents - Discuss the plan for second testing round and share experience on laboratory management focusing on laboratory Quality Assurance and test management. - Staff in charge: Mr. Chris Morrissy, Dr. Long. 2.2. Collect sera samples from 10 provinces for the second round of surveillance, 120 cattle sera and 120 pig sera from each province. - NVDC-Ha Noi: 2 provinces (Quang Ninh and Lang Son), total 480 sera - RAHC-Da Nang: 2 provinces (Kontum and Quang Nam), total 480 sera - RAHC-Can Tho: 2 provinces (An Giang, Kien Giang ), total 480 sera - RAHC-HCMC: 4 provinces (Binh Phuoc, Tay Ninh, Long An, Dong Thap), total 960 sera 2.3 Collection of tissue samples from outbreaks for FMDV serotyping - NVDC-Hanoi: 208 tissue samples from North Vietnam - RAHC-Can Tho: 34 tissue samples from Mekong Delta. - RAHC-HCMC : 390 tissue samples from the South Vietnam . 3. Laboratory Activities 3.1 National Vaterinary Diagnostic Center- Ha Noi (NVDC-Hanoi): - Testing on tissue samples for FMDV serotyping: 208 samples, 7 of them are serotype Asia 1 and 201 of them are Serotype O. - Testing on sera for FMDV antibody detection: 480 sera, on going . 3.2 Regional Animal Health Centre Da Nang (RAHC-Da Nang) - Testing on sera for FMDV antibody detection: 480 sera, on going . 3.3 Regional Animal Health Centre Can Tho (RAHC-Can Tho) - Testing on tissue samples for FMDV serotyping : 34 samples, all of them are serotype O. - Testing on sera for FMDV antibody detection: 480 sera , on going . 3.4 Regional Animal Health Centre HCMC (RAHC-HCMC) - Testing on tissue samples for FMDV serotyping: 390 samples, 1 of them is serotype Asia 1, the remaining are serotype O. - Testing on sera for FMDV antibody detection: 960 sera , on going. - Isolation FMDV by BHK cell line to confirm 3 samples from NVDC- Hanoi, 1 of them is serotype Asia1 in pigs and 1 sample is serotype O and1 sample virus could not isolated. - Isolation FMDV by BHK cell line from 39 selected field samples, 1 was serotype Asia1 and 38 were serotype O. - Application of Virus Neutralisation Test (VNT) protocol (testing phase to setup VNT technology at RAHC-HCMC): • Virus titration for serotype O and A , two viruses for each serotype • VNT applied using the serum from vaccine trial on cattle - RT-PCR application (testing phase to setup molecular technology at RAHC-HCMC, May, 2006) • Extract RNA from virus serotype O, A and Asia 1, two viruses for each serotype • Make cDNA from these RNA • RT-PCR carried out using primers to detect serotype O and A • Sent cDNA and amplicons to AAHL for sequencing Reporter: Ngo Thanh Long Note: Further detail on activities for reporting period in log frame. 5.2 Smallholder Benefits All pig and cattle and producers are potential beneficiaries. Those that take up the advice and use vaccine according to recommendations will benefit financially through reduced losses due to death and disease in their cattle and pigs. The benefits of a more profitable farming operation flow to all family members. Farmers and district veterinarians will have improved knowledge and skill in disease prevention, knowledge on the selection of the right vaccine and improving the efficacy of vaccination 5.3 Capacity Building Training and education of field veterinarians in disease prevention, disease investigation and sample collection has been carried out in the North, Centre and South of Vietnam. This training has already shown an impact with an increase in quality of sample collection and number of samples collected and submitted to the laboratory. The project has provided training and technology transfer of FMD diagnostics to each laboratory involved in the project. Reagents and standard methods have been supplied to each laboratory giving them the diagnostic capability for FMDV diagnosis and serology using ELISA technology, this technology is now able to be practised at each laboratory. 5.4 Publicity The CARD AusAID project have received publicity through the training programs and also through the achievements so far in the project in understanding FMD in Vietnam. FMD is a disease on importance in Vietnam and this has put our project into the lime light. The project has been publicised through a press release in Australia and articles in news letters including the SEAFMD newsletter. 5.5 Project Management Implementation of the project is on schedule. AAHL has kept the project on target and has supported or requests from the Vietnamese partners. Training has been carried out in Australia and Vietnam with both partners benefiting from the information that is being generated. Implementation in Vietnam is also continuing to go well. Trainees have been supplied in a timely manner, from DAH. The field work has been organised well with data and samples being collected from farms in each pilot zone. Training and the transfer of information to other regional laboratories in Vietnam is also occurring. RAHC-HCMC will supply support to these laboratories. 6. Report on Cross-Cutting Issues 6.1 Environment No direct environmental impacts associated with project activities have been identified. On a broader scale, the intention is that pig and cattle farming will become more efficient through a reduction in animal death and disease. Environmental benefit will result through more efficient utilization of resources such as animal feed, the energy to provide animal feed and to run other aspects of the farm. 6.2 Gender and Social Issues Application of new diagnostic tests will improve the ability of regional and provincial diagnostic units to quickly and accurately assess FMD outbreaks, enabling rapid and appropriate measures to be applied to control disease. These benefits will begin during the project and continue to accrue with continued application. The beneficiaries of this project will be both large and small farms and particularly smallholder farmers whose animals and incomes will be protected by better disease diagnosis, management and control. Since women at the village level are the primary animal handlers and managers, they will be major beneficiaries of the final outcome of better diagnosis and control of animal diseases. 7. Implementation & Sustainability Issues 7.1 Issues and Constraints DAH has been very busy controlling the AI outbreaks in Vietnam and this has made there workload increase dramatically, this have meant some delays and rescheduling of training field veterinarians, eg training in the North Vietnam rescheduled. Sampling has also been spread out over a longer time period as well but this has had no effect with all samples still being collected. The collection of samples was made more difficult by the need to train all veterinarians in each province in data collection, on how to collect samples, collection of blood and how to restrain cattle. This increased training need has highlighted the need for further training of field veterinarians throughout Vietnam in disease investigation techniques with a focus on sample collection and data collection. There is a need for further input in this area, large scale training of field veterinarians to ensure better knowledge and control of disease in Vietnam. This could be achieved though better collaboration of aid agencies in Vietnam especially those supplying training for AI. 7.2 Options The government of Vietnam is looking at increasing the support to DAH and has increased funding for AI diagnosis and is looking to do the same for FMD diagnosis. The increased funding for AI has seen laboratories updated with new equipment some of which will improve all disease diagnosis which includes FMD. The money available to improve AI diagnosis can improve all disease diagnosis if this money is used wisely, eg in training field veterinarians in AI diagnosis, the training should be general to cover all diseases including FMD, CSF and other diseases of importance in Vietnam. Also in improving AI diagnosis in laboratories the training should emphasize how improvement can be applied to all areas of diagnosis, eg quality assurance in the laboratory. 7.3 Sustainability The DAH laboratories appear to be well supported by the central government. The DAH laboratories are also receiving increased support from overseas funding agencies as part of the AI campaign to improve laboratory facilities. RAHC-HCMC is developing in confidence as an institution. This confidence is beginning to be passed on to other DAH laboratories. The DAH scientific staff are quick to understand new information and are energetic in its implementation. People have an obvious desire to do their jobs well. This desire is obvious in staff in RAHC- HCMC and other laboratories. The laboratories just need technical support to be encouraged to carry out the techniques that they need for diagnosis and research. The project is following a well tried model for successful technology transfer, of demonstration and teaching of the technology in the donor facility followed by supply of reagents for implementation by the trainees at their own initiative in the recipient laboratory, subsequently backed up by consultancy visits to the recipient laboratory for support and fine tuning. A useful indicator of likely success is the initiative and ability of the trainees to make a useful attempt at implementation by themselves. DAH and NAVETCO staff show a high level of involvement in this way. The AusAID funded CSFV project, that linked DAH, NaVetCo and AAHL prior to this project, is proving sustainable. NAVETCO and DAH not only maintains the diagnostic capability developed at that time, but on its own initiative and unassisted by AAHL is conducting training courses and technology transfer to other labs in Vietnam. 8. Next Critical Steps In the next 6 months a meeting will be held at AAHL to review the project outputs and to examine the information collected so far in the project, The meeting will decide if the data collected from the field is giving DAH the necessary information to make discisions on FMD control. An AAHL empidemilogist will be involved in collating the data from the first 2 phases od serosurveillance and epipdemlogical data collection and begin exposing DAH staff to data analysis techniques. The third phase of the sero-surveillance for the collection of the samples will be implemented with any changes necessary. In the next 6 months we will finalise all of the testing of the samples collected and evaluate each laboratories capabilities. In the second 6 months of the project it was decided to bring forward the training of the staff from each laboratory to be held at AAHL. This training will be carried out at AAHL for 1 representative from each laboratory for training in ELISA techniques. A consultant from AAHL will complete training of the HCMC-RAHC in cell culture techniques and establish this technology there to be used to grow FMD isolates from the field. The growth of FMD isolates from the field is important to allow further analysis of these isolates by PCR and sequencing. 9. Conclusion The project activities are on schedule. The DAH laboratories are running the field trials on time, even though they have had increased workload with avian influenza outbreaks. It is expected that the second sero-surveillance survey will be much easier than the first. The first took a lot longer to do due to commitments with avian influenza and also understanding the problems associated with collecting samples in areas where samples had not been collected before. Field veterinarians needed to be trained in the collection of tissue samples from infected animals as well as bleeding animals. The staff at Regional Animal Health Centre, Ho Chi Min City have performed a excellent job in getting everything to run on schedule in this first six months. The project should continue to run on time and I expect no further problems especially how the collection procedures are in place. The staff at DAH are very eager to learn and this is making a very good environment for transferring technology. The laboratory at Da Nang has less experience and this laboratory will need much more contact with AAHL and the laboratory at RAHC-HCMC to achieve the transfer of the ELISA technology to this laboratory. The laboratory in Hanoi has the skills and is doing quite well in the application of the ELISA but this laboratory needs further contact with AAHL to increase their understanding of the application of this technology for FMD diagnosis and this also applies to the laboratory in Can Tho in the south. Can Tho is applying the technologies learnt. This project has proved to be an important project for Vietnam with the importance of FMD and this has meant good publicity for the AusAID CARD program. It has also meant an increase in requests for help in other areas of animal health with the success of this project in improving the skills and technology at RAHC-HCMC and NAVETCO and through our other project in CSF at NAVETCO and DAH and Duck Plague at NAVETCO. DAH is using this project as an example of how to approach FMD control. 10. Statutory Declaration STATUTORY DECLARATION COLLABORATION FOR AGRICULTURE AND RURAL DEVELOPMENT PROGRAM CARD Project Title: FMD - Improved surveillance capabilities for FMDV CARD Project Number: 072/04VIE We the undersigned hereby declare that during the period 01/06/2005 to 31/12/2005 we have delivered the following inputs to assist in implementation of the above project. 1. PERSONNEL INPUTS Australian Personnel Provided (Name) Days in Vietnam Days in Australia Trips to Vietnam Chris Morrissy (also replaced Laurie Gleeson’s time in project) 28 10 2 Peter Daniels 14 1 Lynda Wright (replaced Brenda Van Der Heide in project ) 14 1 Greer Meehan 10 Axel Colling 5 Catherine Williams 15 Total 56 40 4 Vietnamese Personnel Provided Days in Vietnam Dr.Bui Quang Anh 10 Dr.Hoang Van Nam 20 Dr.Tran Ngoc Thang 15 RAHC-HCMC Dr.Dong Manh Hoa 25 Ngo Thanh Long, DVM 25 Nguyen Truc Ha, Ms 20 Pham Phong Vu, DVM 25 Nguyen Ngo M. Triet, DVM 25 NVDC-HA NOI Dr. To Long Thanh 15 Dr. Nguyen Thu Ha 20 Nguyen Tung, Ms 20 RAHC-DA NANG Tran Van Quan,DVM 15 Le Thanh Quang, DVM 20 Huynh Thi Manh, Ms 20 RAHC-CAN THO Nguyen Ba Thanh, Ms 15 Truong Kim Dung, Ms 20 Tran Quoc Phong, DVM 20 NAVETCO Dr.Tran Xuan Hanh 15 Nguyen Thu Hong, Ms 20 Nguyen Van Hung, Bc 20 2. EQUIPMENT AND OTHER SERVICES Equipment & Other Services Description Budget Limit Inverted Microscope 4000 Pipettes 5000 Microfuge 3000 Laptop 4000 Reagents 4000 Sample Collection 8755 Molecular technologies 2000 Laboratory consumables 4000 Freight 14000 ELIZA work 5000 Project Progress Against Proposed Objectives, Outputs, Activities And Inputs Project Title: Vietnamese Implementing Institution: PROPOSAL PROGRESS REPORT Narrative Information Required Performance Measures Assumptions Information Required OBJECTIVES 1. To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance. (Objective 1.2.2, 2.1.1 & Strategy 1) Samples collected and submitted to laboratory. Data produced and results feedback to field veterinary staff and farms. Technology will be taken up by laboratories. Accurate data collected from farms. Little risk. Establishment of an effective laboratory network for the diagnosis and control of FMD continued with training given at AAHL under a Quality system, method established at each laboratory, QA introduced and resource supplied. The four DAH laboratories are working together to carry out surveillance and testing. 1.1 Train field veterinary staff in outbreak investigation for the investigation of cases of FMD on farms, including record keeping, sampling strategies and data collection. Samples collected from field and submitted to laboratory. Sample and farm records presented with samples. Accurate data collected from farms. Little risk Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff. 1.2 Establish ELISA technology from AAHL to collaborating laboratories Diagnostics test in use. QA controls meet. internal quality control sheets for examination, correct results in external proficiency testing organized by AAHL. Results generated. Technology will be taken up by laboratories. Little risk ELISA training and reagents delivered. Laboratories have began to use the ELISA technology to test samples. Narrative Information Required Performance Measures Assumptions Information Required 1.3 Using cell culture at RAHC- HCMC, make a collection of isolates of FMD viruses from cases of FMD disease on farms. Virus isolates grown in cell culture Cell cultures can be maintained at RAHC- HCMC. Little risk. Training at AAHL carried out and cells transferred to RAHC-HCMC. FMD samples collected. Further training in HCMC this 6 months and cell culture established at RAHC- HCMC 1.4 At RAHC-HCMC and AAHL use molecular techniques to assess strains of FMD circulating Sequence data produced. Technology will be taken up by laboratories. Little risk FMD samples collected 1.5 Introduce cell culture to NVDC- Hanoi for FMD diagnosis Cell culture in use at NVDC-Hanoi Technology will be taken up by laboratories. Little risk Activity later in project 1.6 Firm link with AAHL for the ongoing supply of diagnostic reagents. Diagnostic reagents used for routine diagnostics. Reagents available. Little risk. Process began 2. To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies. (Objective 1.2.2, 2.1.1 & Strategy 1) Project Report presented to DAH and AusAID. Feedback meetings held in provinces. Project objectives meet. Little risk. FMD samples collected Narrative Information Required Performance Measures Assumptions Information Required 2.1 2.2 2.3 2.4 2.5 Hold information and training workshops for farmers and their veterinary advisors, with emphasis on FMD prevention and disease investigation and sample collection Pilot zones established in each province. Feedback to collaborating laboratories results from test comparisons at AAHL and RAHC-HCMC. Feedback results to veterinary field staff and farmers. Determine the efficacy of the FMD vaccine in protecting against FMD isolates circulating in Vietnam. Samples collected from field and submitted to laboratory. Sample and farm records presented with samples. Samples collected from field and submitted to laboratory. Sample and farm records presented with samples. Results on laboratory comparisons and EQA send back to each laboratory. Results maintained at field veterinary office Comparison of FMD genotypes to vaccine. Results of serosurveillance. Accurate data collected from farms. Little risk DAH is able to have farmers agree to project. Little risk Technology will be taken up by laboratories. Little risk Samples collected and tested. Little risk. Samples collected and tested. Little risk. Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff. Further information to farmers to be delivered later in project Pilot zones established. First round of serosurveillance completed. Activity later in project Activity later in project Activity later in project. Samples collected. 2.6 Hold information and training workshops farmers and their veterinary advisors, with emphasis on FMD prevention and presenting the data obtained during the CARD project. Meetings held with farmers and their veterinary advisors Project will achieve objectives. Little risk. Samples collected OUTPUTS 1. & 2. • Field veterinarians that are skilled in on farm diagnosis and sample collection. • Education of farmers to allow better understanding of the benefits of diagnostic technologies. • Development of cell culture capability for virus propagation, isolation from field samples and for serology by virus neutralisation test (VNT). • Diagnostic capabilities at all the laboratories participating in the project to carry out functional tests as described under a Quality System. • Pilot zones established and operational. • Firm link with AAHL for the ongoing supply of diagnostic reagents. • Application of technologies, knowledge and skills to other Samples collected and submitted to laboratory. Data produced and results feedback to field veterinary staff and farms. Technology will be taken up by laboratories. Accurate data collected from farms. Little risk. Process began. Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff. Activity later in project Training at AAHL carried out and cells transferred to RAHC-HCMC. FMD samples collected Establishment of an effective laboratory network for the diagnosis and control of FMD began with training given, method established, QA introduced and resource supplied. Pilot zones established. First round of serosurveillance completed. Process began. Activity later in project 1.1-1.6 & 2.1- 2.6 diseases. • Increased productivity by small farm holders with more food available to poor farmers. This will have a more pronounced effect on the women and children who are mainly responsible for the animals’ upkeep and likely to obtain the major benefits of increased crop production and sale of animals. • Field veterinarians trained in on farm diagnosis and sample collection. • Education of farmers to allow better understanding of the benefits of diagnostic technologies to disease control. • Setup farms/villages in each province for seroservalance • Transfer of ELISA technology to collaborating laboratories Samples collected from field and submitted to laboratory. Sample and farm records presented with samples. Farmers participating in projects Samples collected from field and submitted to laboratory. Sample and farm records presented with samples. Diagnostics test in use. QA controls met, internal quality control sheets available for examination, correct results in external Accurate data collected from farms. Little risk DAH is able to have farmers agree to project. Little risk DAH is able to have farmers agree to project. Little risk Technology will be taken up by laboratories. Little risk Expected outcome at end of project Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff. Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff. Pilot zones established. First round of serosurveillance completed. Establishment of an effective laboratory network for the diagnosis and control of FMD began with training given, method established, QA introduced and resource • ELISA established in each laboratory and EQA carried out in each laboratory • Transfer cell culture capability to RAHC-HCMC • Cell culture used to grow FMDV from field isolates • Transfer molecular techniques for FMDV RT- PCR to RAHC-HCMC • Train RAHC-HCMC staff in sequence analysis of sequenced FMD isolates • Train NVDC in use of cell culture for FMD diagnosis • Communication of diagnostic results to field veterinarians and farmers • Recommendations on efficacy of FMD vaccines proficiency testing organized by AAHL. Results generated. Results on laboratory comparisons and EQA send back to each laboratory. Virus isolates grown in cell culture. FMDV isolates sequenced PCR products send to AAHL Sequence data produced. Trainee at AAHL from NVDC-Hanoi Results maintained at field veterinary offices Comparison of FMD genotypes to vaccine. Technology will be taken up by laboratories. Little risk Cell cultures can be maintained at RAHC- HCMC. Little risk. Cell cultures can be maintained at RAHC- HCMC. Little risk. Technology will be taken up by laboratories. Little risk PCR products submitted to AAHL Trainee does not attend training. Little risk Samples collected and tested. Little risk. Samples collected and tested. Little risk. supplied. Process began. Training at AAHL carried out and cells transferred to RAHC-HCMC. FMD samples collected Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project • Comparison of FMD isolates circulating in Vietnam • Reagents in use in DAH laboratories and purchase of reagents by DAH for ongoing epidemiology studies and serosurveillance. • Final project report written summarising results obtained from project • Report to DAH and results of project to be published Results of serosurveillance. Report on sequence analysis of FMD isolates Diagnostic reagents used for routine diagnostics. Meetings held with farmers and their veterinary advisors. Final reports written. FMD Isolates collected. Little risk. Reagents available. Little risk Project will achieve objectives. Little risk. Project will achieve objectives. Little risk. Activity later in project Activity later in project Activity later in project Activity later in project ACTIVITIES 1. & 2. To establish an effective laboratory network for the diagnosis and control of FMD by the provision of resources and training of staff in required methods and quality assurance. To provide accurate data to explain failure of vaccination to control FMDV and to develop new effective vaccine application strategies. . Major activity/objective of the project, to be completed over the 3 years of the project Diagnostic capabilities at all the laboratories participating in the project to carry out functional tests as described under a Quality System. Field veterinarians that are skilled in on farm diagnosis and sample collection. Education of farmers to allow better understanding of the benefits of diagnostic technologies. Pilot zones Establishment of an effective laboratory network for the diagnosis and control of FMD began with training given, method established, QA introduced and resource supplied. Pilot zones established. First round of serosurveillance completed. • Order and purchase equipment • Supply laboratory consumables • AAHL staff will prepare information and lectures for workshop for training and education of field veterinarians and farmers • Run first workshop at RAHC- HCMC field veterinarians • Supply consumables and budget for sample collection • RAHC-HCMC to initiate epidemiological and serosurveillance programs • RAHC-HCMC and NCDC- Hanoi to coordinate training and meetings with field First month year 1 First 3 months year 1 First 3 months year 1 First 3 months year 1 First 3 months year 1 First 3 months year 1 First 3-6 months year 1 established and operational. Recommendations on vaccine application from epidemiological and serosurveillance data. Equipment in place at laboratories Consumables at laboratories Workshop held at RAHC-HCMC Workshop held at RAHC-HCMC Consumables and budget supplied Contact made with field veterianians to begin sample collection for disease outbreaks Workshop & meetings held Equipment in place at laboratories Consumables supplied to laboratories Completed. Information supplied and used in workshops Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff. Consumables and budget supplied Training carried out in the North, Centre and South of Vietnam for veterinary field and laboratory staff. veterinarians in provinces where fields studies are to be carried out. • Hold meetings to educate farmers on the benefits of epidemiological studies, serosurveillance and laboratory diagnostics to disease control • Sampling schedule setup for each province in the project • Farms/village setup with information on sampling schedule and project requirement • Costs of sampling negotiated with farmers and field veterinary staff • Serosurveillance carried in each province • Samples collected from field and tested in each laboratory • Scientists from RAHC-HCMC laboratories to be trained in First 3-6 months year 1 First month year 1 First 3 months year 1 First 3 months year 1 Twice a year for 3 years Twice a year for 3 years First 3 months year 1 Meetings held Document produced for sampling schedule Document for project field studies circulated to filed veterinarians and farmers Payment details completed Samples collected and send to laboratories Results generated Scientists trained at AAHL Initial contact made with farmers in project. Education to be carried out as part of National program Sampling schedule documented during workshop for Veterinarians. Pilot zones established. First round of serosurveillance completed. Pilot zones established. First round of serosurveillance completed. Budget for field work received and transfer to field staff. Completed FMD outbreak samples tested by ELISA. Activity to continue later in project Completed FMD diagnostics, ELISAs and cell culture at AAHL. • Follow up training by AAHL to establish ELISA and cell culture techniques at RAHC- HCMC. • Cell cultures and consumables supplied to RAHC-HCMC • Cell cultures grown and stored at RAHC-HCMC • Cell cultures used for virus isolation and serology • AAHL to run ELISA workshop at RAHC-HCMC • Follow training by RAHC- HCMC staff to each laboratory • FMD ELISAs established in regional laboratories. • Samples send from each laboratory to RAHC-HCMC and AAHL for comparison • Accreditation of laboratory for FMD ELISAs First 3 - 6 months year 1 First 3 - 6 months year 1 First 3 - 6 months year 1 7-8th month of year 1 First 6 months year 1 First 3 - 6 months year 1 First 6-9 months year 1 Twice a year for 3 years First 6-9 months year 1 Visit by AAHL consultant to RAHC- HCMC Cell cultures & consumables delivered Cell cultures in use FMDV isolated from field SNT used for serology Workshop completed ELISA working in each laboratory ELISA results send to AAHL Results of comparisons reported back to laboratories Activity later in project Completed Completed Activity later in project Completed Completed Activity later in project Activity later in project Activity later in project • One scientist from each of the regional laboratories trained in ELISA techniques at AAHL. • Visit by AAHL scientist to each regional laboratory to check ELISA operation and QA in the laboratories • Carry out EQA testing at each laboratory • Meeting to be held in Australia with project leaders to discuss progress of project, QA lectures will be part of this visit • Set up external QA program for the regional laboratories at RAHC-HCMC. • RAHC-HCMC scientists trained in molecular techniques • PCR products FMD isolates in Vietnam sent to AAHL from RAHC-HCMC • Sequencing on FMDV First 1 month year 2 First 3 months year 2 First 3 months year 2 Middle of Year2 First 3 months year 3 Third 3 months of Year 2 Second & Third year of project Second & Third year of ELISAs functional at each laboratory, IQC available Scientists trained at AAHL Visit by AAHL consultant completed EQA results collated and returned to each laboratory Carry out final round of EQA Scientists trained at AAHL PCR products received at AAHL Sequence data from FMD isolates produced Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project isolates collected in Vietnam at AAHL • AAHL staff to train regional laboratory staff in analyzing data from the field • One NVDC-Hanoi scientist trained at AAHL in cell culture. • Scientist from RAHC-HCMC to be trained in cell culture and virus isolation at AAHL • Supply cells to HVDC-Hanoi • Further workshops at regional centres to reinforce disease investigation protocols and to provide feedback to veterinarians and farmers on the epidemiological and serosurveillance data collected. Review of project and accomplishments. • Finalise results from samples from field outbreaks and report back to field veterinarians and farmers project First 3 months year 3 First 3 months year 3 First 3 months year 3 First 3 months year 3 Last 6 months year 3 Twice a year for 3 years AAHL staff to visit Vietnam Scientist trained at AAHL Scientist trained at AAHL Cell cultures received at HVDC-Hanoi Feedback to field veterinarians and farmers on project results and recommendations Results reported to DAH and farmers Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project • Finalise serology results and report back to field veterinarians and farmers • Summarise results from epidemiological studies and serosurveillance from project • Carry out sequencing of FMD isolates collected during project • Write recommendations for use of FMD vaccine • Draft final project report • Write final project reports for CARD and present report to MARD and DAH Twice a year for 3 years Last 6 months year 3 Second & Third years of project Last 6 months year 3 Last 3 months year 3 Last 3 months year 3 Results reported to DAH and farmers Write report Collate & report sequence data from project Report recommendations from project Final project meeting held Reports completed Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project Activity later in project INPUTS See attached statuary declaration and above for project inputs